Help! PCR Problems

Yang Hsiang Mei linp.bbs at gopher.ncku.edu.tw
Mon Nov 7 22:45:54 EST 1994


Hi Netters,
   I'm having trouble amplifying an 11kb fragment from total genomic mice DNA.
My PCR result has always been a large smear. I followed the TaKaRa Ex Taq
kit's protocol with some modifications (temp/time combinations). My
primers were 30bp & 33bp long. Does anyone have better experiences in doing
PCR using the total genomic DNA as template, or amplifying large fragments?
Any help would be greatly appreciated!





More information about the Methods mailing list