Help! PCR Problems
Yang Hsiang Mei
linp.bbs at gopher.ncku.edu.tw
Mon Nov 7 22:45:54 EST 1994
I'm having trouble amplifying an 11kb fragment from total genomic mice DNA.
My PCR result has always been a large smear. I followed the TaKaRa Ex Taq
kit's protocol with some modifications (temp/time combinations). My
primers were 30bp & 33bp long. Does anyone have better experiences in doing
PCR using the total genomic DNA as template, or amplifying large fragments?
Any help would be greatly appreciated!
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