How to Calculate Oligo MW?

Stephen R. Lasky, Ph.D. alt.binaries.pictures.erotica.blondes alt.binaries.pictures.erotica.d alt.binaries.pictures.erotica.female alt.binaries.pictures.erotica.male alt.binaries.pictures.erotica.orientals alt.binaries.pictures.fine-art.d alt Stephen_Lasky at brown.edualt.fan.hello-kittyalt.fan.hofstadteralt.fan.holmesalt.fan.howard-sternalt.fan.howard-stern.fartmanalt.fan.hurricane.yipalt.fan.itchy-n-scratchyalt.fan.james-bondalt.fan.jello-biafraalt.fan.jen-
Mon Nov 7 09:05:19 EST 1994


In article <N.L.Brown.1134506748B at news.bham.ac.uk>, N.L.Brown at bham.ac.uk
(Nigel Brown) wrote:

> In Article <1994Nov3.140526.1 at mcvax4.d48.lilly.com>,
> books at mcvax4.d48.lilly.com wrote:
> >We are looking for a formula to calculate the exact MW for oligonucleotides.
> >The oligo will have a 5'-hydroxy group. We can estimate oligonucleotide MW
> >if we use 330 as the average MW for each base but we need a exact number. 
> >Thanks DPSMITH smith_dennis_p at lilly.com
> 
> GOOD GRIEF!  I have no objection to helping people with technical and other
> problems of a specialist nature, but are there really people doing science
> who cannot calculate a molecular weight?  No wonder
> bionet.molbio.methds-reagnts is becoming overloaded.

As I suspected, you have started getting people flaming you (gently so
far) for posting this statement.  I'd just like to say that I agree with
you and that people should think about their questions a little before
posting them to this group.

SRLasky

-- 
*********************************************************************Stephen R. Lasky, Ph.D.
Roger Williams Medical Center/Brown University
Phone: 401-456-6572       Fax: 401-456-6569       e-mail: Stephen_Lasky at brown.edu
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"To me at least, 'Yuck' doesn't capture the full essence of death by
neurotoxin."   -Dick Dunn
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