RNaseA activity at elevated temp.?

Tracy Aquilla aquilla at salus.med.uvm.edu
Mon Nov 7 17:05:46 EST 1994


In Article <39dlpg$hqt at hermod.uio.no>, ohungnes at bioslave.uio.no (Olav
Hungnes) wrote:
>The RNase A is able to survive high temperatures.
>I'm curious to know how high temperatures we can have 
>in our digestion and still have reasonable activity.
>
>In other words, my requirement is that the enzyme shall 
>be usefully active, not only survive, at this temperature.
>
>Olav
>--
>_______________________________________________________
>Olav Hungnes                 olav.hungnes at embnet.uio.no
>National Institute               Phone  (+47)22042200
>of Public Health                 FAX    (+47)22353605
>Oslo, NORWAY
>_______________________________________________________

When I make solutions of RNAse A, I boil them for ten minutes and then
quench on ice. I don't know at what temperature the activity begins to
decrease, but I would guess RNAse A would work even in a PCR reaction. It
would be very easy for you to test this yourself.
    Tracy



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