Heparin in PCR?

Shahram Mori smori at nmsu.edu
Sun Nov 6 21:49:23 EST 1994


macklon at acs.ucalgary.ca wrote:
: In article <393iuv$fhk at owl.csrv.uidaho.edu>, raman913 at uidaho.edu
: (Ramanujam Raman) wrote:


: I'd like to add another question regarding heparin: I've heard that it can
: be an inhibitor in PCR rxns., which I assume is due to its DNA mimicry.  I
I have heard that people use heparinase to get rid of the heparin prior to
PCRing.
Cheers,
--
Shahram Mori					   _/\_
Program in Molecular Biology			  _\  /_
Dept. of chemistry and Biochemistry Box 3C	  \_  _/
NMSU  Las Cruces NM				    ||
88003





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