Flagging or hexaHis tag?
r.james at uea.ac.uk
Mon Nov 7 11:12:54 EST 1994
Should we stick to what we know and use the flag epitope
> (an 8 amino peptide against which these exists a monoclonal antibody)
> or should we try the His-6 tag and Ni chromatography for purification.
We have used the His-Tag method to purify several proteins now and
have had no trouble with any of them. It seems to work really well.
You can even use it to purify protein complexes by putting the
His-Tag on one protein and then denature on the Ni-column to purify
I am a fan of this system.
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