Human cell line with inducible lacZ gene
mrclean at u.washington.edu
Tue Nov 8 12:22:53 EST 1994
Has anyone created a human cell line possessing a single copy of the E.
coli lac Z gene (under the control of an inducible promoter) in its
genome. We are particularly interested in cell lines where lacZ gene
expression is tightly regulated (if such a thing is possible in a
mammalian cell...); in other words, the basal level of lacZ gene
transcription is very low or undetectable in the absence of induction.
We would like to utilize such a cell line as a positive control in some
experiments we are planning. We would, or course, cover the cost of
shipping a sample of the cells.
Thanks in advance for any information or help you can provide.
Please respond directly to: mrclean at u.washington.edu
Department of Microbiology
Mail stop SC-42
University of Washington
Seattle, WA 98195
Phone: (206) 543-8440
FAX: (206) 543-8297
email: mrclean at u.washington.edu
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