What is 'gel mobility shift assay'?

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In article <94311.215150VHWBC at CUNYVM.CUNY.EDU>, D. P. Wijesinghe
<VHWBC at CUNYVM.CUNY.EDU> wrote:

> Could someone please let me know what 'gel mobility shift assay' is?
>  
> Harshinee Wijesinghe

It is an technique that allows the visualization of proteins, usually from
nuclear extracts, that bind to specific DNA sequences.  A short segment
of  DNA is labeled with 32p and incubated with the extract contaning DNA
binding proteins.  The mixture is then run out on a low percentage
non-denaturing polyacrylamide gel.  If the DNA is bound by proteins, its
mobility is slowed on the gel (it shifts in mobility from the free probe).
Hence the name.

SRL

-- 
*********************************************************************Stephen R. Lasky, Ph.D.
Roger Williams Medical Center/Brown University
Phone: 401-456-6572       Fax: 401-456-6569       e-mail: Stephen_Lasky at brown.edu
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