What is 'gel mobility shift assay'?

Stephen R. Lasky, Ph.D. alt.binaries.pictures.erotica.blondes alt.binaries.pictures.erotica.d alt.binaries.pictures.erotica.female alt.binaries.pictures.erotica.male alt.binaries.pictures.erotica.orientals alt.binaries.pictures.fine-art.d alt Stephen_Lasky at brown.edualt.fan.hello-kittyalt.fan.hofstadteralt.fan.holmesalt.fan.howard-sternalt.fan.howard-stern.fartmanalt.fan.hurricane.yipalt.fan.itchy-n-scratchyalt.fan.james-bondalt.fan.jello-biafraalt.fan.jen-
Tue Nov 8 11:12:48 EST 1994


In article <94311.215150VHWBC at CUNYVM.CUNY.EDU>, D. P. Wijesinghe
<VHWBC at CUNYVM.CUNY.EDU> wrote:

> Could someone please let me know what 'gel mobility shift assay' is?
>  
> Harshinee Wijesinghe

It is an technique that allows the visualization of proteins, usually from
nuclear extracts, that bind to specific DNA sequences.  A short segment
of  DNA is labeled with 32p and incubated with the extract contaning DNA
binding proteins.  The mixture is then run out on a low percentage
non-denaturing polyacrylamide gel.  If the DNA is bound by proteins, its
mobility is slowed on the gel (it shifts in mobility from the free probe).
Hence the name.

SRL

-- 
*********************************************************************Stephen R. Lasky, Ph.D.
Roger Williams Medical Center/Brown University
Phone: 401-456-6572       Fax: 401-456-6569       e-mail: Stephen_Lasky at brown.edu
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