What is 'gel mobility shift assay'?
doerther at merle.acns.nwu.edu
Tue Nov 8 11:26:26 EST 1994
In article <94311.215150VHWBC at CUNYVM.CUNY.EDU>, D. P. Wijesinghe
<VHWBC at CUNYVM.CUNY.EDU> wrote:
> Could someone please let me know what 'gel mobility shift assay' is?
> Harshinee Wijesinghe
A gel mobility shift assay, or gel shift, is used to determine
Protein/DNA interactions. For example, one may synthesize an oligo with a
restriction factor binding site, mix protein + 32P labelled oligo, and run
on an acrylamide gel. Once you have been able to shift a band, in future
experiments you can compete away signal by mixing the previously mentioned
"soup" with excess unlabelled oligo to prove Protein/DNA interaction via
competition. Hope this helps. Most, if not all, Molecular Biology
Protocol manuals will have a more complete description of the procedure.
More information about the Methods