stabilty of biotinylated primers

Frederick Garbrecht FRED at bmt.mcw.edu
Tue Nov 8 09:35:47 EST 1994


> To:            methods-and-reagents at net.bio.net
> From:          Douglas.Lyonns at net.bio.net
> Subject:       stabilty of biotinylated primers
> Reply-to:      baharvey at swansea.ac.uk
> Date:          Tue, 8 Nov 1994 10:35:07 GMT

> Help!
> does anyone know what happens to biotinylated primers if you store
> them at 4'C. We have got a feeling that all the biotin falls off.
> Can any one confirm this?
> Thanks, 
> Doug
> 
> 
The biotin moiety should be covalently linked to the oligonucleotide, 
so it cannot just fall off.  If you are having problems, it is more 
likely that your oligo is degrading under your particular storage 
conditions.

Frederick C. Garbrecht
fred at bmt.mcw.edu
Bone Marrow Transplant Program
Medical College of Wisconsin
phone 414 257 5053
fax   414 257 7994



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