stabilty of biotinylated primers

Frederick Garbrecht FRED at
Tue Nov 8 09:35:47 EST 1994

> To:            methods-and-reagents at
> From:          Douglas.Lyonns at
> Subject:       stabilty of biotinylated primers
> Reply-to:      baharvey at
> Date:          Tue, 8 Nov 1994 10:35:07 GMT

> Help!
> does anyone know what happens to biotinylated primers if you store
> them at 4'C. We have got a feeling that all the biotin falls off.
> Can any one confirm this?
> Thanks, 
> Doug
The biotin moiety should be covalently linked to the oligonucleotide, 
so it cannot just fall off.  If you are having problems, it is more 
likely that your oligo is degrading under your particular storage 

Frederick C. Garbrecht
fred at
Bone Marrow Transplant Program
Medical College of Wisconsin
phone 414 257 5053
fax   414 257 7994

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