Dumb question (?) re cycle sequencing of PCR products

(Dave Johnston) daj at mailserver.nhm.ac.uk
Tue Nov 8 05:38:38 EST 1994


Hi,
When cycle sequencing PCR products, the dogma seems to be that you can't 
use either of the original PCR primers as a sequencing primer. Why not? 
They bind and act as primers for second strand synthesis by Taq (or the PCR 
wouldn't work in the first place), what more do you need? Is it just a 
scam to get us to buy more oligos or am I missing something?

Thanks
DAJ

David A. Johnston
Research Fellow,
Dept of Zoology, The Natural History Museum, Cromwell Road,
South Kensington, London SW7 5DB. England
(tel 071 9389297, fax 071 9388754, email daj at nhm.ac.uk)



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