GST fusion prot.- HELP

[Kevin Pumiglia]

In article <39m95b$q7t at steele.ohsu.edu>, nishir at edu.ohsu (Rae Nishi) wrote:

> We have had good luck reducing degradation by growing and inducing bugs
> carrying the GST expression vector at 30 degrees instead of 37 degrees.
> 
> Rae Nishi
> Oregon Health Sciences University
> Portland, OR
> nishir at ohsu.edu

Likewise, we have found with GST-constructs which are easily degraded
during induction, that inducing at room temperature O/N  decreases the
degradation.