help sequence from puc

macklon at acs.ucalgary.ca macklon at acs.ucalgary.ca
Tue Nov 8 20:14:50 EST 1994


In article <carmean-0811941114350001 at creslab2.biol.sfu.ca>, carmean at sfu.ca
(Dave Carmean) wrote:

> I would like to receive copies of protocals for standard sequencing of
> double-stranded DNA.  I am having difficultlies getting good sequence from
> puc and I'd like to compare your methods with ours to help pinpoint the
> problem.
> 
> William D. Brown
> Department of Biological Science
> Simon Fraser University
> Burnaby, B.C.  V5A 1S6
> Canada

I use the Sequenase (USB Biochemicals) kit for sequencing double-stranded
DNA from plasmid preps (mini or regular) with great success. Could be your
preps aren't quite clean enough, in which case RNase treatment might
help.  
bb (beckthol at acs.ucalgary.ca)



More information about the Methods mailing list