help sequence from puc

macklon at macklon at
Tue Nov 8 20:14:50 EST 1994

In article <carmean-0811941114350001 at>, carmean at
(Dave Carmean) wrote:

> I would like to receive copies of protocals for standard sequencing of
> double-stranded DNA.  I am having difficultlies getting good sequence from
> puc and I'd like to compare your methods with ours to help pinpoint the
> problem.
> William D. Brown
> Department of Biological Science
> Simon Fraser University
> Burnaby, B.C.  V5A 1S6
> Canada

I use the Sequenase (USB Biochemicals) kit for sequencing double-stranded
DNA from plasmid preps (mini or regular) with great success. Could be your
preps aren't quite clean enough, in which case RNase treatment might
bb (beckthol at

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