MDBK electroporation

peter s. evans evans at ahabs.wisc.edu
Tue Nov 8 16:50:23 EST 1994


Hi!

Would anyone out there care to share conditions, parameters, and/or
experiences on the electroporation of Madin-Darby Bovine Kidney (MDBK)
cells?  Such information would be very helpful.  I am currently trying to
optimize electroporation using transient transfection with a lacZ carrying
plasmid as a measure of transfection efficacy.  I am using the Invitrogen
electroporator II.

Reply by e-mail, or post.  Thanks (in advance) for your help :-)

Pete Evans



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