Homemade GeneClean kits ???
mbxspd at unicorn.nott.ac.uk
Tue Nov 8 06:58:55 EST 1994
We make and use our own home-made 'Gene-clean' kits on a regular basis
here. The original reference we have is Vogelstein & Gillespie (1979)
Proc. Natl. Acad. Sci Vol.76(2) pp615-619.
Our protocol is an adaptation of the one described in this paper.
The solutions we use are:
1) 6M NaI, 0.1% sodium thiosulphate
2) 4.5M NaI, 0.1% sodium thiosulphate
3) TE, pH8, 100mM NaCl, 70% absolute ethanol
We store all solutions at 4¡C.
For the glass 'beads', we use a finely crushed scintillation vial i.e
high quality glass.
All reagents in the above solutions were of the highest quality
available. This is especially important for the NaI. Using the above
reagents we havn't ever needed to filter the NaI solutions through
glass fiber filters.
If you require a detailed protocol, drop me a line via email and I will
send you one :)
Queens Medical Centre,
University of Nottingham,
Simon Dawson Internet email: mbxspd at unicorn.nott.ac.uk
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