Sterilization a viscous medium

Thanit Pewnim thanip at NWG.NECTEC.OR.TH
Wed Nov 9 18:21:15 EST 1994


	I am preparing Medium 199 containing 3% (w/v) chondroitin sulfate 
for keeping corneas. Sterilization using 0.22 micron membrane is rather 
difficult due to the viscous nature of chondroitin sulfate. Any 
suggestions out there for alternative methods?

					Many Thanks, TP
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 THANIT PEWNIM..> Department of Chemistry, Silpakorn University,
	          Nakornpathom 73000, THAILAND, Facimile +66 34 255820
         	  Telephone +66 34 255797, Internet <thanip at nwg.nectec.or.th>
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