Oligo Labeling With Terminal Transferase
lippay at vaxd.gat.com
Wed Nov 9 19:06:11 EST 1994
In a recent hybridization experiment we have been labeling 17-mer
degenerate oligos with 32P dATP using Terminal Transferase. One set of
oligos is similar except it is shifted three bases and the 3' end
terminates with a C in one and an A in the other. We seem to be getting
2-3 times higher dATP incorporation in the oligo which ends in a C. Does
anyone know if TdT prefers a certain 3' base over another when labeling
oligos? If so, this should be incorporated in your strategy when designing
probes. Thanks for the info.
General Atomics Bioscience Div.
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