FRED at bmt.mcw.edu
Wed Nov 9 09:02:22 EST 1994
> To: methods-and-reagents at net.bio.net
> From: grggta at picr.cr.man.ac.uk (Graham Atherton)
> Subject: Antibody warning
> Date: 9 Nov 1994 13:08:38 -0000
> A well known Californian company recently sold us an antibody. We did
> several experiments on cellular protein and got no specific bands of
> the expected size. We thus phoned up the company who then admitted that
> they had never got the antibody to work on cell extracts, only on bacterially
> expressed protein! We have thus wasted 2-3 weeks of precious time and
> extract. They are sending a PAN-antibody in the hope it will help free of
> charge - but we are not getting our money back.
> Always take care to ask as many questions about newly-made antibodies
> before committing your resources!
> Graham Atherton
Care to tell us which company and which antibody?
Frederick C. Garbrecht
fred at bmt.mcw.edu
Bone Marrow Transplant Program
Medical College of Wisconsin
phone 414 257 5053
fax 414 257 7994
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