help with laarge plasmids

M.A.Schmidt infekt at
Wed Nov 9 04:59:47 EST 1994

Hi out there,
we are in need of a good reliable methods for the isolation of large
plasmids (> 150 kb) from E.coli. We have already tried a number of
techniques but nothing came of it. Does anyone know or has experience with
these large plasmids and could help us with a suggestion? It would help us
quite a lot and would be really appreciated! With many thanks 
M.Alexander Schmidt

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