non-radioactive RNAse protection assays

Patrice Ferriola Ferriola at ciit.org
Wed Nov 9 16:46:31 EST 1994


Does anyone have any ideas about non-radioactive detection methods
for RNAse protection assays?  I presently use 33P or 35S-lableled
riboprobes, but would like to make a probe with longer shelf life.
Is there any way to label the riboprobe with fluorescent nucleotides and then detect the protected/fluorescent bands directly on a urea gel?  I don't want to transfer the gel.  Applied Biosystems couldn't
provide any answers.  Any ideas?

thanks



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