help:how tomake 250ug 130bp fragment?

David W. Plank plank001 at maroon.tc.umn.edu
Wed Nov 9 14:13:00 EST 1994


On Thu, 03 Nov 1994 21:55:04 +0200, 
Dana Wolle  <wolle at biochem.purdue.edu> wrote:

>   I need 250ug of a 130bp DNA fragment for affinity column. I don't know
>what is the goog method to make it. Anybody can help?
>
>  lu at aclcb.purdue.edu
>
 
I assume your looking for a method to make the affinity column not a 
method to purify the fragment.  You can make the affinity column by 
reacting your DNA fragment directly with cyanogen bromide activated 
Sepharose 4B.

References:

   Pang, R., et. al. Biochem. Biophys. Res. Commun. 67, 508 (1975)
   Revel, M., et. al. Methods in Enzymol. 79, 149 (1981)
   Wagner, et. al. Biochem. Biophys. Res. Commun. 454, 184 (1971)
   Schmuckler, M. et. al. J. Biol. Chem. 250, 2206 (1975)
   Levy, C. et. al. J. Biol. Chem. 248, 5712 (1973)
   Yarbrough, L. and J. Hurwitz, J.Biol.Chem. 249, 5396 (1974)

Good luck and watch those typo's damn it.

Best Regards,
Dave






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