gene sequencing-HELP

[Colin Rasmussen]

> I'm new in molecular biology.  I would like to sequence  a 300 pb fragment using 
> the dideoxy method. Everything is OK except when I want to recuperate the gel to 
> put it in the gel dryer. The gel sticks on the plate and when I peel the 
> blotting paper up off the plate, the gel never comes with it! what can I do?
> Thank you very much.
> email: roquet at ljcrf.edu


Try using Sigma-Cote to treat the gel plates, it will help you get the 
gel off.

Colin