PCR question

Grad Student Lab 1 gslab1 at unixg.ubc.ca
Wed Nov 9 22:13:52 EST 1994


 Hi, there. Here is a question about PCR in my lab: if the amount of one 
primer in the reaction is more than that of another one, let's say 30% more, 
what will happen? Will the product mainly be a single strand headed with the 
majority primer? For instance, if the majority primer is 1.1 kb far away from 
the 5' end of its template, will the major product of this PCR be a 1.1 kb 
DNA? 
	Any direction and comments are welcome.

Please directly reply here or e-mail me at  xzhou at unixg.ubc.ca



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