Karyotype protocol for amphibian cell line

Rachel bub rachelbub at aol.com
Fri Nov 11 01:40:05 EST 1994

I am looking for a way to karyotype an amphibian cell line.  The current
protocol which I am using is out of the newest edition of Freshney. 
However, their given protocol does not give great results, possibly
because of the difference between  mammalian cell culture conditions and
amphibian culture conditions.  Anyone have any advice to offer?  Please
Email directly if possible.  Thanks!!!

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