Electroporators: what brand?

Anthony Tomlinson tomlinson at pplros.demon.co.uk
Thu Nov 10 06:45:09 EST 1994


In article <39dp8j$rfd at cronkite.ocis.temple.edu> ,
mike at sgi1.fels.temple.edu writes:
>I'm looking for advice on what type of Electroporator to get.  I'll be 
>transfecting E. coli and vertebrate cells.  So far I'm looking at the
Bio-Rad 
>(seems to be the standard) and the BTX.  Any experience with either? 
Also, any 
>other brands to recommend?
>Thanks:	mike at sgi1.fels.temple.edu

I use the Bio-Rad Gene Pulser for E coli, and it works fine.
 Reproducible, reliable, fairly robust (it doesn't keel
 over if you blow up the occasional cuvette), but the cuvettes
 are pricey.  Some people reuse 'em, but I've been too
 lazy so far.
 
 Hope this helps
 
 Anthony



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