Phage DNA

Todd Johnson TJOHNSON at scinet.weber.edu
Thu Nov 10 11:26:50 EST 1994


I tried to post this message yesterday but I lost it in the internet
black hole.  Regarding the extraction of phage DNA, I think you need
to include a proteinase K digestion step  after the PEG precip.  I
have always done this before Phenol/chloroform extraction.  Typically
the digestion is done at 50C for 1 to 2 hr.

TRY IT! and IT WILL COME

good luck
Todd Johnson**********************************************************************

Todd M. Johnson, Ph.D.
Chemistry Dept.
Weber State University

E-mail:  TJohnson at scinet.weber.edu

**********************************************************************



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