help:how tomake 250ug 130bp fragment?

G G
Fri Nov 11 05:29:55 EST 1994


In article <wolle-031194215504 at bc214a.biochem.purdue.edu>, wolle at biochem.purdue.edu (Dana Wolle) says:
>
>   I need 250ug of a 130bp DNA fragment for affinity column. I don't know
>what is the goog method to make it. Anybody can help?
>
>  lu at aclcb.purdue.edu
What you need to do is to repeatedly PCR the fragment. You can get
maybe a microgram of product from a single PCR reaction. Therefore
250 PCR reactions will give you what you want.



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