5'-end labeling of 8mer oligo?

pzhang pzhang at cc.UManitoba.CA
Fri Nov 11 19:46:46 EST 1994


Hi, netters,
I posted my broblem a couple of days ago. Having got no reply, I'd like to try again.
I want to radioactivly label 5'-end of a 8mer oligo. I am not sure how to separate labled oligo from the reaction (removal of ATP, ADP etc.) I want to use this oligo as a strand of an adapter. So, my second question is: If I use the labeled reaction directly( without any purification) to go ahead, what are the ligation results?

Any ideas and suggestions are highly appreciated.

Peijun Zhang
pzhang at cc.umanitoba.ca



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