help with laarge plasmids

Prenom Nom Prenom.Nom at rsvs.ulaval.ca
Fri Nov 11 06:34:42 EST 1994


In article <infekt-0911941059470001 at ifimac04.uni-muenster.de>,
infekt at uni-muenster.de (M.A.Schmidt) wrote:

> Hi out there,
> we are in need of a good reliable methods for the isolation of large
> plasmids (> 150 kb) from E.coli. We have already tried a number of
> techniques but nothing came of it. Does anyone know or has experience with
> these large plasmids and could help us with a suggestion? It would help us
> quite a lot and would be really appreciated! With many thanks 
> M.Alexander Schmidt

We isolated large (200kb) plasmid from Agrobacterium tumefaciens with the
method of Currier and Nester.  ref.: Currier T. and Nester E.W, 1976. 
Anal. Biochem. 76:431-441.

Hope it helps

           Christian



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