Xenopus oocytes/expression/DNA injection nucleus

[Andreas Buhr]

Hi netters,

I would like to ask if someone has experience with DNA injection into the
nucleus of Xenopus oocytes. 

Experiments with a dye indicated that we can inject into the nucleus with
90% probability. Next, we did an experiment injecting 10 nl DNA in 5 mM
HEPES pH 7.4 ( 0.01 to 10 ng DNA) into the nucleus. The constructs have a
CMV promoter/enhancer and a SV40 Poly A. After 3 days we found in 2/10
oocytes  2 myA using 10 ng DNA. The other amounts didn't expressed
currents.

Any comments and advice is appreciated

Posted this article also in bionet.neuroscience

Andreas Buhr
Pharmakologisches Institut
Universitaet Bern
Switzerland

Email:  Buhr at pki.unibe.ch