ABI 373 DNA sequencer

Torben Osterlund torben.osterlund at medkem.lu.se
Sat Nov 12 08:45:03 EST 1994


In article <39qt17$oef at mserv1.dl.ac.uk> Jacqueline Vanderstappen,
jacqueline.vanderstap at agr.kuleuven.ac.be writes:
>We've recently bought the upgrade version (34cm WTR). Sometimes we have 
>problems with directly sequencing dsPCR products with the Taq dye
terminator 
>kit. It's not a problem of reactions because the sequencing with the
same 
>reactions have worked with the previous version of ABI 373 (24cm WTR).
Is 
>this a problem of the gel (other concentration) or/and of the running 
>conditions. We now run the samples at 30 W. 
>Is this too high for PCR products?

Hi.
An important thing to check in each run, is the voltage at start, since
this changes considerably during the run. Our experience is that you
should change the Wats between 20-30 W untill the voltage shows 1200.
Also always prerun your gel for 10 min to equilibrate it.



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