help w/ ecor1 contam!
smori at nmsu.edu
Fri Nov 11 23:10:56 EST 1994
Mandalaparthy (padmmv00 at ac.usfca.edu) wrote:
: Dear Netters,
: This may seem like the most bizarre thing, but the Eco R1 enzyme and
: buffer we have seem to be contaminated leading to DNA degradation problems
: repeatedly in our lab. We are using absolutely sterile tips, tubes and
: distilled water.If any generous soul out there has any clue as to what may
: be happening I would be really grateful!!!!!!.Even the brand new stuff is
: degrading absolutely good batches of both plasmid and lambda gt10 dna
: preps. I am so frustrated and would really thank anyone who has any help
: or suggestions.
: THANKS IN ADVANCE>Please e-mail to Padmmv00 at ac.usfca.edu.
: Padmaja Mandalaparthy.
: Frustrated Grad student
: USF, CA.
Try changing the company that you order the enzyme from. The whole batch
that they may have produced might be contaminated.
Shahram Mori _/\_
Program in Molecular Biology _\ /_ Saskatoon/SK/CANADA
Dept. of chemistry and Biochemistry Box 3C \_ _/
NMSU Las Cruces NM ||
More information about the Methods