Help! Western problems!

Kevin Pumiglia pumiglk at
Sat Nov 12 08:00:08 EST 1994

In article <lank-mrc.1134975152C at>, lank-mrc at
        100 (lmrc.) wrote:

> I have nonspecific bands showing up on my Western that looks identical to
> those stained by Ponsau S.  I tried varying milk %, but still not much
> improvement.  I tried different antibodies, and still the same problems. 
> What's going on?  How could all (3) of the different antibodies that I have
> used be "bad"???  Help!   
> lank-mrc at

They probably are not!  Chances are it is your secondary detection reagent
(Protein-A/ 2nd Ab).  Try titering this out and see if the background

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