Help! Western problems!
pumiglk at aa.wl.com
Sat Nov 12 08:00:08 EST 1994
In article <lank-mrc.1134975152C at tigger.jvnc.net>, lank-mrc at tigger.jvnc.net
100 (lmrc.) wrote:
> I have nonspecific bands showing up on my Western that looks identical to
> those stained by Ponsau S. I tried varying milk %, but still not much
> improvement. I tried different antibodies, and still the same problems.
> What's going on? How could all (3) of the different antibodies that I have
> used be "bad"??? Help!
> lank-mrc at tigger.jvnc.net
They probably are not! Chances are it is your secondary detection reagent
(Protein-A/ 2nd Ab). Try titering this out and see if the background
More information about the Methods