Sequencing Gel Fixation

Yasha Hartberg Yasha at bioch.tamu.edu
Sun Nov 13 16:41:04 EST 1994


In article <tsute.267.000B56F6 at microbio.umass.edu>,
tsute at microbio.umass.edu (Tsute Chen) wrote:

 > I've heard that the fixation step (with 10% acetic acid & 10% methanol)
can be 
 > skipped without much difference in the result during the normal Sanger's
DNA 
 > sequencing procedures. Does anyone have any experience or opinion about
this?

This one has gone around several times.  The verdict is:  stop wasting your
time fixing gels!

Yasha Hartberg
Texas A&M University
"If people want to spend their whole lives creaming and tweezing and
brushing and tilting and gluing, that's really okay too, because it gives
them something to do."  Andy Warhol



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