Sequencing Gel Fixation

[Tsute Chen]

I've heard that the fixation step (with 10% acetic acid & 10% methanol) can be 
skipped without much difference in the result during the normal Sanger's DNA 
sequencing procedures. Does anyone have any experience or opinion about this?


Tsute Chen                  E-mail: tsute at microbio.umass.edu
Department of Microbiology  
University of Massachusetts  
Box 35720 
Amherst, MA 01003-5720