Sequencing Gel Fixation

Tsute Chen tsute at
Sun Nov 13 11:20:18 EST 1994

I've heard that the fixation step (with 10% acetic acid & 10% methanol) can be 
skipped without much difference in the result during the normal Sanger's DNA 
sequencing procedures. Does anyone have any experience or opinion about this?

Tsute Chen                  E-mail: tsute at
Department of Microbiology  
University of Massachusetts  
Box 35720 
Amherst, MA 01003-5720

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