Help! Western problems!
smori at nmsu.edu
Sun Nov 13 23:11:26 EST 1994
lmrc. (lank-mrc at tigger.jvnc.net100) wrote:
: I have nonspecific bands showing up on my Western that looks identical to
: those stained by Ponsau S. I tried varying milk %, but still not much
: improvement. I tried different antibodies, and still the same problems.
: What's going on? How could all (3) of the different antibodies that I have
: used be "bad"??? Help!
: lank-mrc at tigger.jvnc.net
Try using Gelatin for your wash instead of milk. It's much better.
Shahram Mori _/\_
Program in Molecular Biology _\ /_ Saskatoon/SK/CANADA
Dept. of chemistry and Biochemistry Box 3C \_ _/
NMSU Las Cruces NM ||
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