! ! ! ! ! H E L P ! ! ! ! !
smori at nmsu.edu
Sun Nov 13 23:17:12 EST 1994
Graeme Mulvaney (byh003 at coventry.ac.uk) wrote:
: HELP ! ! ! !
: I am a final year biochemistry student in Coventry University, for
: my project I have to Isolate Alkaline phosphatease from Thermus aquaticus.
: Can anybody help me ?
: I am looking for assistance with growing the Bacterium, isolating and
: purfying the enzyme and assaying the enzyme!
: If you have any experiance working with extremely thermophilic bacteria
: then PLEASE mail me ! ! !
: My life is in your hands (well sort of !)
: Thank you ! !
: Graeme Mulvaney
: P.S. My project starts in 3 weeks time !!
Have you considered using antibody to the protein coupled to a sepharose
column? Break cells open, ammonium sulphate fractionated, and run the
fractions over the column. The protein will bind to the column.
Shahram Mori _/\_
Program in Molecular Biology _\ /_ Saskatoon/SK/CANADA
Dept. of chemistry and Biochemistry Box 3C \_ _/
NMSU Las Cruces NM ||
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