Sequencing Gel Fixation

Stephen R. Lasky, Ph.D. alt.binaries.pictures.erotica.blondes alt.binaries.pictures.erotica.d alt.binaries.pictures.erotica.female alt.binaries.pictures.erotica.male alt.binaries.pictures.erotica.orientals alt.binaries.pictures.fine-art.d alt Stephen_Lasky at brown.edualt.fan.hello-kittyalt.fan.hofstadteralt.fan.holmesalt.fan.howard-sternalt.fan.howard-stern.fartmanalt.fan.hurricane.yipalt.fan.itchy-n-scratchyalt.fan.james-bondalt.fan.jello-biafraalt.fan.jen-
Mon Nov 14 12:54:19 EST 1994


In article <tsute.267.000B56F6 at microbio.umass.edu>,
tsute at microbio.umass.edu (Tsute Chen) wrote:

> I've heard that the fixation step (with 10% acetic acid & 10% methanol)
can be 
> skipped without much difference in the result during the normal Sanger's DNA 
> sequencing procedures. Does anyone have any experience or opinion about this?
> 
> 
> Tsute Chen                  E-mail: tsute at microbio.umass.edu
> Department of Microbiology  
> University of Massachusetts  
> Box 35720 
> Amherst, MA 01003-5720

We never fix our gels anymore although, as you are likely to hear, not
doing so can lead to sticky gels.  We found that if you dry the gel for an
hour at 70 deg C and then continue drying for 10 or 15 min's without heat
that the gels aren't sticky.  

SRLasky

-- 
*********************************************************************Stephen R. Lasky, Ph.D.
Roger Williams Medical Center/Brown University
Phone: 401-456-6572       Fax: 401-456-6569       e-mail: Stephen_Lasky at brown.edu
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"To me at least, 'Yuck' doesn't capture the full essence of death by
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