PCRing 5' UTR using a cDNA plasmid?

Mari L. Shinohara Mari.L.Shinohara at dartmouth.edu
Mon Nov 14 21:40:09 EST 1994


Hi folks,

I need to amplify 5' untranscribed region from genomic (total) DNA. 
However, I now only have a cDNA plasmid of the gene of interest.  I
heard that there are some ways to do it by using anchor(s) (not really
RACE in this case).  Could you send me any protocols and/ or ideas? 
Your help will be appreciated!

Mari



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