5' RACE Specificity Problems
childs at oci.utoronto.ca
Mon Nov 14 23:11:46 EST 1994
I'm having trouble with specificity in 5'RACE.
I'm getting non-specific bands, even though my gene-specific
primer is an 18 mer with a Tm of 58C, I'm doing the PCR with a hot start and
annealing at 56C. At the same time I am not getting the product I want.
Has anyone had similar problems and overcome them? Does anyone have a
very good RACE protocol that might help me?
Ontario Cancer Institute
email: childs at oci.utoronto.ca
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