The Case of the Vanishing Intron

[Thierry Nouspikel]

In article <39uaih$1rv at arcadia.informatik.uni-muenchen.de> 
salger at wap18.zi.biologie.uni-muenchen.de (Klaus Salger) writes:

>Taq has some reverse transcriptase activity. I found two
>references about that.
>...
>Maybe you want to treat your DNA with RNase to exclude this possibility?

Well, thanks for your suggestion, Klaus. I may try using RNAse although I 
don't really believe in this explanation: if the unexpected band in the 
genomic PCR was due to RNA contamination I should also see a minor, misspliced 
band. I don't see why only the correctly spliced product would 
contaminate/amplify?

>If  you find the explanation, please post it! 
I will... provided I find one.



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Thierry Nouspikel, MD                 ¦ "Un technocrate c'est un mec,
Genetics & Microbiology Dpmt          ¦ tu lui poses une question,
University of Geneva, Switzerland     ¦ quand il a fini de répondre
nouspike at cmu.unige.ch                 ¦ tu comprends plus ta question."
fax:41/22/329.34.04                   ¦ Michel Coluci, dit "Coluche"