Help! Western problems!

Anne Spang spang at vms.biochem.mpg.de
Mon Nov 14 10:39:56 EST 1994


In article <lank-mrc.1134975128B at tigger.jvnc.net>,
lank-mrc at tigger.jvnc.net wrote:

Did you also try different secondary antibodies? You could try different
blocking reagents and mixtures of them e.g. 5% milk 5% ovalbumin.
Sometimes it helps, if you add detergent such as Tween 20 at 0.1% up to
0.5%. Are you sure that your buffers are not contaminated?

Hope this helps!

Anne



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