RNA from archival/fixed tissue
shopkins at fs1.ho.man.ac.uk
Tue Nov 15 16:48:01 EST 1994
Has anyone had any success with recovering RNA from histological specimens?
We would like to recover low copy number mRNA (for cytokines) from fixed
tissue sections for RT-PCR, but have had no success. We have tried methods
based on protracted incubation with Proteinase K, or microwaving with 'Gene
Releaser' (Cambio), but have obtained either nothing or some very degraded RNA.
More information about the Methods