CAT assays (in cell culture media)

Martin Leach leach at mbcrr.harvard.edu
Tue Nov 15 11:54:35 EST 1994


In article <kaufmann-141194160919 at 129.194.99.105>, kaufmann at cmu.unige.ch
(J. Kaufmann) wrote:

> I perform my CAT assays by diffusion of acetylated products in the
> scintillation cocktail during the CAT assay, and another problem is the
> volumes of media that I have to use. Extraction is much less efficient in
> 1ml of medium compared to 30l of cell extract.
> 
> Has anybody tried to perform CAT assays in cell media? 

I know 5' to 3' (the company) makes a CAT elisa where you could measure CAT
protein in the cell media...and overcome any inhibitory effects on the CAT
enzyme..

Martin Leach

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