CAT assays (in cell culture media)

Martin Leach leach at
Tue Nov 15 11:54:35 EST 1994

In article <kaufmann-141194160919 at>, kaufmann at
(J. Kaufmann) wrote:

> I perform my CAT assays by diffusion of acetylated products in the
> scintillation cocktail during the CAT assay, and another problem is the
> volumes of media that I have to use. Extraction is much less efficient in
> 1ml of medium compared to 30l of cell extract.
> Has anybody tried to perform CAT assays in cell media? 

I know 5' to 3' (the company) makes a CAT elisa where you could measure CAT
protein in the cell media...and overcome any inhibitory effects on the CAT

Martin Leach


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