DOT BLOTS using BIOTINYLATED probes

Jim Burrows jburrows at nwu.edu
Tue Nov 15 16:58:29 EST 1994


Has anyone had any luck using biotinylated DNA probes to detect target DNA
blotted onto membrane?  We had a probe made with biotin at both the 3' and
5' ends and HPLC purified but we cannot get detection with strep-avidin.  

Please e-mail me with any suggestions and I will post anything that works.

Thanks in advance,

-- 
Jim Burrows
jburrows at nwu.edu



More information about the Methods mailing list