Need large, intact human genomic clones?
ung at cco.caltech.edu
Tue Nov 15 18:03:40 EST 1994
For those of you who would like to obtain human genomic DNA pieces
in the form of stable, easily manipulated clones in E. coli hosts,
a BAC (Bacterial Artificial Chromosome) library is available.
Average insert size of the library is about 125 kb, and the library
covers more than 90% of human genome. The clones are stably propagated
as a recombinant F-factor in a recombination-deficient E. coli host
strain. The library can be screened by almost any kind of probes including
cDNA, oligos, other genomic clones (plasmid, cosmid, lambda, YAC etc).
We will screen the library to find out BAC clones covering the regions
of your intesrest, and if necessary, will also develop contig maps that
depict overlap-relationships among the clones covering the same loci.
All it takes will be a letter of collaboration.
Interested party should contact:
Division of Biology,
Pasadena, CA 91125
ung at ash.tree.caltech.edu
More information about the Methods