Phage DNA preparation

Steven Goldberg goldberg at
Tue Nov 15 14:52:35 EST 1994

I found that I obtained poor phage growth on plates using either LB bottom agar or an agarose overlay.  I use NZY medium (which contains added Mg++) and NZY top agar.  For phage DNA preparation I use liquid cultures as described in BioTechniques vol. 14, p. 360 (1993).  PEG, SDS, and DNase are not used so the DNA usually digests very well.

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