Phage DNA preparation

Steven Goldberg goldberg at bms.com
Tue Nov 15 14:52:35 EST 1994


I found that I obtained poor phage growth on plates using either LB bottom agar or an agarose overlay.  I use NZY medium (which contains added Mg++) and NZY top agar.  For phage DNA preparation I use liquid cultures as described in BioTechniques vol. 14, p. 360 (1993).  PEG, SDS, and DNase are not used so the DNA usually digests very well.



More information about the Methods mailing list