New DNA stain
gshearer at whale.st.usm.edu
Wed Nov 16 15:56:47 EST 1994
Robert Hindges (hindges at vetbio.unizh.ch) wrote:
: Hi Netters
: Recently I have seen a picture of a agarose gel, where the DNA was stained
: with a substance called SybrGreen. It is not toxic and it only stains the
: DNA, not the gel, therefore it is much nicer to work with than with EtBr.
: Does anybody knows more about this stain, i.e. where to buy or how much it
We've used SybrGreen. Its an interesting dye...
Essentially no background thus no need to destain.
If you use shortwave UV _on top_ of the gel (epi-illumination)
it is quite a bit more sensitive than EtBr.
The down side is that it is expensive. (About $200/ml)
But as I recall, the working dilution is 1/5,000 or something
in this neighborhood, and it can be reused for a week or so.
Also, I'm not aware that there is any good toxicity or mutagenicity
data available. Are you sure that it is 'non-toxic'?
[just a customer]
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