Long & Accurate PCR

[Thierry Nouspikel]

Hello collegues,

I gathered there are now companies selling "kits" for long and accurate 
PCR (for instance TaKaRa did a lot of advertisement recently).

Does anybody have experience with these? What product would you recomend ?
My goal is to amplify a ~4Kb mRNA by RT-PCR, to clone it and to seach the 
clones for mutations. Thus is has to be accurate: I don't want to sequence 
half a dozen of clones to tell the mutation(s) from PCR-introduced errors.

Thanks for any advice,

                                     Thierry Nouspikel

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Thierry Nouspikel, MD                 ¦ "Un technocrate c'est un mec,
Genetics & Microbiology Dpmt          ¦ tu lui poses une question,
University of Geneva, Switzerland     ¦ quand il a fini de répondre
nouspike at cmu.unige.ch                 ¦ tu comprends plus ta question."
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