unable to ppt. protein using ammonium sulphate

Wed Nov 16 17:14:06 EST 1994

I have a problem trying to precipitate a purified protein, I have 
used almost 60% of ammonium sulphate and actually the solution is 
saturated and I still don't see any cloudiness. I'm sure that the 
concentration of the protein is at least about 15ug/ml and I can see 
a band on SDS gel when I loaded 10ul of the sample.  I heard that 
usually 45% of it will do the job! and I don't know what the story is
about my situation. Would someone who has an idea of this
situation gives me some advices please? and thanks in advance!


Benson Laboratory
Department of Microbiology
Microbiology Building
University of Maryland
   at College Park
College Park, M.D. 20742
Email  sablab at microb.umd.edu
Phone  301-405-5441
Fax    301-314-9489

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