Small scale RNA /DNA preps

Elise Lamar Lamar at sc2.salk.edu
Wed Nov 16 20:38:04 EST 1994


I am looking for a method to use on small amounts of tissue, i.e.,
embryos, limb buds, tail tips, etc., to make a crude prep of RNA for RT
PCR along with a bit of genomic DNA to use for genotyping by PCR.  I want
to use this method on small preps of muscle tissue derived from
transgenic mice to test for expression of the transgene, but it is
important that I be able to genotype the samples. Does anyone have a
protocol or a helpful reference.



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